Immature Embryo-Derived of Two Bread Wheat (Triticum aestivum L.) Varieties Transformation Using Particle Bombardment Method
نویسندگان
چکیده
منابع مشابه
Genetic Diversity and Population Structure Analysis of European Hexaploid Bread Wheat (Triticum aestivum L.) Varieties
Progress in plant breeding is facilitated by accurate information about genetic structure and diversity. Here, Diversity Array Technology (DArT) was used to characterize a population of 94 bread wheat (Triticum aestivum L.) varieties of mainly European origin. In total, 1,849 of 7,000 tested markers were polymorphic and could be used for population structure analysis. Two major subgroups of whe...
متن کاملRetraction of article: Stable chloroplast transformation of immature scutella and inflorescences in wheat (Triticum aestivum L.).
Dear editor; After receiving the comments from the readers, we checked the results carefully and decided to repeat all the experiments. As the first author has already left the lab, other members are still trying to repeat these experiments. Because it takes a long time to have these experiments redone, we decide to retract our paper entitled “Stable chloroplast transformation of immature scute...
متن کاملStable chloroplast transformation of immature scutella and inflorescences in wheat (Triticum aestivum L.).
Chloroplast transformation in wheat was achieved by bombardment of scutella from immature embryos and immature inflorescences, respectively. A wheat chloroplast site-specific expression vector, pBAGNRK, was constructed by placing an expression cassette containing neomycin phosphotransferase II (nptII) and green fluorescent protein (gfp) as selection and reporter genes, respectively, in the inte...
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This report describes the delivery of plasmid DNA into wheat anther culture derived embryos. Initial electroporation experiments were conducted using enzyme (NT-1) pretreatment, 500 F capacitance, and plasmid pAM2100, which carries the herbicide resistance gene, bar, and the screenable marker gene, Gus. Electroporated embryos were allowed to recover 10 days on regeneration medium and then trans...
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The objectives of this study were to compare nonparametric stability measures, and to identify promising high-yield and stable bread wheat (Triticum aestivum L.) genotypes in 7 environments during 2003-2005 in the central Black Sea region of Turkey. The bread wheat genotypes (20 advanced lines and 5 cultivars) were grown in a randomized complete block design with 4 replications in 7 different e...
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ژورنال
عنوان ژورنال: Annual Research & Review in Biology
سال: 2014
ISSN: 2347-565X
DOI: 10.9734/arrb/2014/8499